|
KMID : 1161520130170020121
|
|
Animal Cells and Systems
2013 Volume.17 No. 2 p.121 ~ p.126
|
|
|
Immunomodulatory effects of Clostridium butyricum on human enterocyte-like HT-29 cells
|
|
Gao Quanxin
Qi Lili
Wu Tianxing
Xia Tingting
Wang Jinbo
|
|
|
Abstract
|
|
|
|
Oral administration of Clostridium butyricum as probiotics is increasingly gaining importance in the treatment of intestinal inflammations and improvement of animal performance. The mechanisms of host cell receptor recognition of C. butyricum and downstream immune signaling pathways leading to this beneficial event, however, remain unclear. In this study, C. butyricum was investigated for its capability to influence the innate immune response of HT-29 cells to explore its mechanism of action. Our data showed that C. butyricum was able to stimulate toll-like receptor 2 (TLR2) production at mRNA level; however, TLR4, TLR5, TLR9, and myeloid differentiation primary response protein 88 (MyD88) transcription levels remain unaltered. The nuclear factor κB (NF-κB), interleukin-8 (IL-8), and tumor necrosis factor alpha (TNF-α) levels in response to C. butyricum were significantly increased, indicating that HT-29 cells were sensitised by C. butyricum. It is the first time that our findings have showed the specific signaling pathways in HT-29 cells stimulated by C. butyricum, which, at least in part, can help to explain the beneficial properties of C. butyricum.
|
|
|
KEYWORD
|
|
|
Clostridium butyricum, HT-29 cells, NF-kB/IkB, toll-like receptors
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|